John Shackleford
- Date of birth: about 1700
- Place of birth: Virginia
- Date of death: ?
- Father: James Shackleford
- Mother: Elizabeth Robins
- Marriage: Ann Lyne (date of marriage before 1725)
(daughter of John Lyne, had brother named George)
- Children:
- James
- Lyne
- Benjamin
- Samuel
- John
- Richard
- Zachariah
- William